Did it come with a cue sheet? If so, open that cue sheet with Notepad or some text editor and tell me if you see the data track listed twice, once in the beginning and once at the end. I think 3DO games were the ones where they had that type of weird layout.
3DO is strictly one track only. No audio, no second data track, no nothing. Also, because of the odd 3DO format you can't view the files in the ISO (assuming it's cooked) like you can with say a SegaCD or Saturn 2048/cooked ISO image.
For some reason the only prog I could get to make a working 3DO disc from a 2048 ISO is DiscJuggler 3.xx. If the image isn't 2048/cooked you should be able to burn it with CDRWin. The nifty lil' trick is figuring out which format your ISO is in without a CUE sheet. If you don't mind wasting a CDR or two you could use the example CUE that lockecole2 just posted - that's about how a 2352/RAW 3DO CUE would look - just adjust the file name and CUE sheet to match each other.